Custom TaqMan Gene Expression Assays

Each custom assay is a mix of forward primer, reverse primer, and FAM or VIC dye-labeled TaqMan MGB probe. Designed to run under universal oligo concentration and thermal cycling conditions for two-step RT-PCR, these assays are simple to use. Just add TaqMan Master Mix and your cDNA sample to generate sensitive, reproducible, and truly quantitative RNA expression data.

There are two custom assay options to meet your RNA research needs: Custom TaqMan Gene Expression Assays and Custom Plus Applied Biosystems TaqMan RNA Assays. Both allow you to submit a target sequence to our TaqMan Assay design pipeline, which uses your input sequence to design custom primer and probe sequences. See the key differences between the two options below:

Feature	Custom Plus TaqMan RNA Assay	Custom TaqMan Gene Expression Assay
Bioinformatic analysis performed on input target sequences
In silico QC performed on assay sequences
Specificity option for gene-level or transcript-level detection
Contains TaqMan MGB probe and 2 unlabeled primers
Runs under universal thermal cycling conditions
Assay sequences provided
Available for any species	*Available for any species with genome information in our database
Automatic check for predesigned TaqMan Assays which may detect input sequence

Custom Plus TaqMan RNA Assays are designed using the full bioinformatic power of the TaqMan Assay design pipeline, which uses proprietary algorithms that have generated millions of assay designs and is regarded as the benchmark in the industry for qPCR design. If you do not need bioinformatics or QC checks performed on your sequences, then our standard Custom TaqMan Gene Expression Assays will meet your needs.

Target sequence submission is easy using the Custom Assay Design Tool (CADT). Simply select your bioinformatic analysis and assay specificity preferences, and enter your target sequence. Sequence submissions are completely confidential; we will not share your target sequences or assay sequences with any third parties.

If you do not have a pre-defined input sequence, the CADT will help you to search for sequences by keyword (gene symbol, accession number, etc.) or by genomic location in a broad range of the most popular and well-studied model species ranging from human and mouse to pig, dog, cow, Arabidopsis, and more.

1. Bergmann O et al. (2011) Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. Exp Cell Res 2, 188-194.
2. Hevir N, Sinkovec J, Rizner TL (2011) Disturbed expression of phase I and phase II estrogen-metabolizing enzymes in endometrial cancer: lower levels of CYP1B1 and increased expression of S-COMT. Mol Cell Endocrinol 1, 158-167.
3. Colazzo F et al. (2011) Extracellular matrix production by adipose-derived stem cells: implications for heart valve tissue engineering. Biomaterials 1, 119-127.